Lethal effect of Silver Nanoparticles Synthesized with Essential Oils of Syzygium aromaticum and Cymbopogon citratus on Tetranychus urticae Koch (Acari: Tetranychidae)
Paper ID : 1059-IPCA5 (R1)
Authors
Kimia Zamanlou, Solmaz Azimi *, Parisa Lotfollahi, Saeed Mollaei
Azarbaijan Shahid Madani University
Abstract
One of the important pests of agricultural crops is the two-spotted spider mite. Although some essential oils and plant extracts have shown desirable insecticidal properties against this pest, these compounds have been less practical and commercialized due to their volatility and instability. Therefore, in recent years, nanotechnology has attracted the attention of researchers as a factor in the durability of plant essential oils. In this study, the effect of two essential oils, clove (Syzygium aromaticum) and lemongrass (Cymbopogon citratus), and nanoparticles synthesized by silver nitrate were evaluated. GC-MASS was used to identify the chemical compositions of the essential oils. The laboratory condition was determined at photoperiod 16 hours of light and 8 hours of darkness, humidity 60±5 percentages and temperature 27±2 ºC. The results showed that the main components of the studied essential oils were monoterpene compounds. The LC50 values of contact toxicity, clove essential oils, clove nano formulation, lemongrass and lemongrass nano formulation were 61.73, 46.96, 97.53 and 52.64 microliters/ml, respectively. Comparison of the slopes of the lines based on the parallelism hypothesis showed that there was a significant difference between the slopes of the probit mortality lines of clove essential oils, clove nano formulation, lemongrass, and lemongrass nano formulation. Based on the results, clove essential oil has a higher contact toxicity against the adult stage of the two-spotted spider mite than lemongrass essential oil. Also, comparing the LC50 of lemongrass nano formulation with the LC50 of clove nano formulation showed that there was no significant difference in the contact toxicity of these two essential oils.
Keywords
clove, GC-MASS, LC50, lemongrass, nano formulation, toxicity, two-spotted spider mite.
Status: Abstract Accepted