| How different liquid media affect blastospore production of Beauveria bassiana; A comparative study on pathogenicity against Tetranychus urticae under laboratory conditions |
| Paper ID : 1065-IPCA5 (R1) |
| Authors |
|
Katayoon kheradmand * University of Tehran |
| Abstract |
| Entomopathogenic fungi play a vital role in managing pests, particularly in controlling the two-spotted spider mite, Tetranychus urticae Koch. In the present study, possible effects of different supplementary compounds on the blastospore production of entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. Strain OZ1 were investigated under laboratory conditions. As the first step, effects of extracts of different agricultural products including “rice bran”, “wheat bran”, “barley bran”, “maize bran”, and “potato waste” on the blastospore production were evaluated. The results obtained revealed that the highest sporulation rate was in medium containing “barley bran”. Then, different supplementary proteins including “whey”, “soybean”, and “egg white” with a concentration of 4% were added into the medium enriched with “barley bran”, and their effects on blastospore production were assessed. The results obtained in this step highlighted the significant effect of “whey protein” on the blastospore production of B. bassiana. In the last step, different chloride salts (“KCl”, “MgCl2”, and “CaCl2”) were added to the medium fortified by “barley bran+whey protein” and their effects on the sporulation of B. bassiana was evaluated. The data gathered in this step indicated that the use of “potassium chloride (KCl)” had the highest impact on the blastospore production than other salts tested. Finally, a bioassay procedure was run to compare the pathogenicity of blastospores obtained from liquid medium “barley bran+whey protein+ KCl” and conidia harvested from solid culture against the two-spotted spider mite, Tetranychus urticae Koch. No statistical difference was detected when compare the pathogenicity of blastospore obtained in liquid medium (LC50 = 6.86 × 105 Blastospore/ml) with conidium produced in solid medium (LC50 = 7.68 × 105 Conidium/ml). This information underlined the effectiveness of liquid media for developing mass rearing programs of B. bassiana. |
| Keywords |
| Agricultural pests, Bicontrol agents, Entomopathogenic fungi, Natural media, Pathogenic spores, Spider mites |
| Status: Abstract Accepted |
