| The effect of inducing compounds on bean plants in absence and presence of Tetranychus urticae Koch, 1836 (Trombidiformes: Tetranychidae) |
| Paper ID : 1140-IPCA5 (R2) |
| Authors |
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Atefeh Dahmardeh1, malihe latifi *2, Roohollah Saberi riseh2 1Agriculture, Vali-e-Asr University of Rafsanjan 2Department of Plant Protection, College of Agriculture, Vali-e-Asr University of Rafsanjan |
| Abstract |
| The ability of plants to increase the expression of agents involved in defense mechanisms against pests is known as ‘induced resistance’’. This process can be activated further by various external factors like pathogens, pests, elicitors of biotic origin, abiotic elicitors and chemical products. In the present study, we assay the role of some inducing compounds (The selection of these materials was based on previous experiments) in the activation of the defense mechanism of the bean plant against Tetranychus urticae Koch. To rear the two-spotted spider mite, green bean seeds were planted in pots under controlled conditions (25±1°C temperature, 70±5% relative humidity, and 16:8 h light-dark photoperiod). At the two-leaf stage, plants were infested with female mites, and after three generations, mites were collected for laboratory experiments. To examine the effects of inducing compounds (potassium phosphite, amino acid complex, and biolog siderophoric (resistance inducing factor) extracted from Pseudomonas fluorescens), bean plants were grown to the four-leaf stage, and 100 ml of each treatment was added to the soil. Two female mites were then placed on each plant. Sampling was conducted at day 0 and on days 5, 10, and 15 after treatment. Leaves were frozen in liquid nitrogen and stored at -80°C. Enzyme extracts were prepared to measure the levels of peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia lyase (PAL). Additionally, 20 days after treatment, the levels of chlorophyll, carotenoids, and total phenolic compounds were measured in four plant groups: treated-infested, treated-uninfested, untreated-infested, and untreated-uninfested. The results showed that inducing compounds increased the level of polyphenol oxidase, phenylalanine ammonia lyase, peroxidase, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids, leading to the activation of the plant’s defense system. Our data also showed that mite infestation increased the level of enzymes, even in plants untreated with inducing compounds. Results of this study suggest that inducing compounds could be a sustainable and environmentally-friendly replacement to chemical pesticides in the near future. |
| Keywords |
| Defence; Induce resistance; Two spotted spider mite |
| Status: Abstract Accepted (Poster Presentation) |
